Discrimination of Dendropanax morbifera via HPLC fingerprinting and SNP analysis and its impact on obesity by modulating adipogenesis- and thermogenesis-related genes

Dendropanax morbifera (DM), a medicinal plant, is rich in polyphenols and commonly used to treat cancer, inflammation, thrombosis. However, date, no study has been conducted on DM regarding the enormous drift of secondary metabolites plants different regions Republic Korea their effects antiobesity, explore compounds that play an important role two major obesity-related pathways. Here, we present in-depth samples collected from three [Jeju Island (DMJ), Bogildo (DMB), Jangheung (DMJG)]. We high-performance liquid chromatography (HPLC) multivariate component analyses analyze polyphenol contents (neochlorogenic acid, chlorogenic cryptochlorogenic rutin), followed by discrimination DMJG using single nucleotide polymorphism chemometric analysis. In silico vitro evaluation found plant extract anti-obesity pathways (adipogenesis thermogenesis) was carried out. Furthermore, extraction methods (Soxhlet ultrasound-assisted extraction) were understand which method better why. Upon quantifying with polyphenols, had highest content polyphenols. The internal transcribed region (ITS) revealed specific gel-based band for authentication DMJG. PCA PLS-DA polyphenol’s discriminative power extracts related contents, showing effect DMJ DMB. Ultrasound-assisted yielded high number compared Soxhlet method, supported scanning electron microscopy. work encourages studies efficiently use them dietary therapeutic purposes.